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Comparison of Different Mass-Spectrometry Workflows for the Proteomic Analysis of Tear Fluid

ABSTRACT: Proteomic analysis of tear fluid is challenging mainly due to a wide dynamic range of proteins and small sample volume. However, recent advancements in mass spectrometry have revolutionized the field of proteomics through its unprecedented depth, speed, and accuracy even with small sample volumes. In this study using the Orbitrap Fusion Tribrid mass spectrometer, we compared four different mass-spectrometry workflows for proteomic analysis of human tear fluid.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Tear

SUBMITTER: Ashok Sharma

LAB HEAD: Ashok Sharma

PROVIDER: PXD030990 | Pride | 2022-04-04

REPOSITORIES: Pride

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Comparison of Different Mass Spectrometry Workflows for the Proteomic Analysis of Tear Fluid.

Jones Garrett G Lee Tae Jin TJ Glass Joshua J Rountree Grace G Ulrich Lane L Estes Amy A Sezer Mary M Zhi Wenbo W Sharma Shruti S Sharma Ashok A

International journal of molecular sciences 20220219 4

The tear film is a multi-layer fluid that covers the corneal and conjunctival epithelia of the eye and provides lubrication, nutrients, and protection from the outside environment. Tear fluid contains a high concentration of proteins and has thus been recognized as a potential source of biomarkers for ocular disorders due to its proximity to disease sites on the ocular surface and the non-invasive nature of its collection. This is particularly true in the case of dry eye disease, which directly ...[more]

PMID: 35216421

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microRNA expression data from human corneal epithelial cells exposed to media, human tear, media + P. aeruginosa antigens, tear + P. aeruginosa antigens

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