Identification of ETV6 bound protein by affinity purification of ETV6-HA fusion protein in REH cells

ABSTRACT: To determine the protein partners of ETV6, we expressed ETV6 and HA-tagged ETV6 in Reh pre-B leukemic cells. Anti-HA magnetic beads were used for affinity purification of ETV6-HA (and ETV6 control) from the nuclear and cytoplasmic fraction. Following purification on-beads digest and LC-MS/MS experiments were performed at the Proteomics platform of the CHU de Quebec Research Center, Quebec, Canada.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Cell Culture

DISEASE(S): Acute Leukemia

SUBMITTER: Alain Bataille  

LAB HEAD: Daniel Sinnett

PROVIDER: PXD031001 | Pride | 2022-01-26

REPOSITORIES: Pride