Identification of ETV6 bound protein by affinity purification of ETV6-HA fusion protein in REH cells
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ABSTRACT: To determine the protein partners of ETV6, we expressed ETV6 and HA-tagged ETV6 in Reh pre-B leukemic cells. Anti-HA magnetic beads were used for affinity purification of ETV6-HA (and ETV6 control) from the nuclear and cytoplasmic fraction. Following purification on-beads digest and LC-MS/MS experiments were performed at the Proteomics platform of the CHU de Quebec Research Center, Quebec, Canada.
INSTRUMENT(S): TripleTOF 5600
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): B Cell, Cell Culture
DISEASE(S): Acute Leukemia
SUBMITTER: Alain Bataille
LAB HEAD: Daniel Sinnett
PROVIDER: PXD031001 | Pride | 2022-01-26
REPOSITORIES: Pride
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