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Effects of contact allergens on THP-1 cells – LFQ- and SILAC-based untargeted proteomics

ABSTRACT: In this study, the effects of the contact allergens BQ, DNCB, NBB, Ni, and PPD were investigated in THP-1 cells. For this purpose, LFQ- and SILAC-based untargeted proteomics were applied.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Isabel Karkossa

LAB HEAD: Kristin Schubert

PROVIDER: PXD031017 | Pride | 2023-09-13

REPOSITORIES: Pride

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A comparative proteomics analysis of four contact allergens in THP-1 cells shows distinct alterations in key metabolic pathways.

Höper Tessa T Karkossa Isabel I Dumit Verónica I VI von Bergen Martin M Schubert Kristin K Haase Andrea A

Toxicology and applied pharmacology 20230803

Allergic contact dermatitis (ACD) is the predominant form of immunotoxicity in humans. The sensitizing potential of chemicals can be assessed in vitro. However, a better mechanistic understanding could improve the current OECD-validated test battery. The aim of this study was to get insights into toxicity mechanisms of four contact allergens, p-benzoquinone (BQ), 2,4-dinitrochlorobenzene (DNCB), p-nitrobenzyl bromide (NBB) and NiSO<sub>4</sub>, by analyzing differential proteome alterations in T ...[more]

PMID: 37541627

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