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Carnosine effect in scaffold free human dermis spheroids model

ABSTRACT: Carnosine is an endogenous β-alanyl-L-histidine dipeptide endowed of antioxidant and carbonyl scavenger properties, able to significantly prevent the visible signs of aging and photoaging. To deeply investigate the mechanism of action of carnosine on human skin proteome, a 3D scaf-fold-free spheroids model of primary dermal fibroblasts from 50-years-old donor, was here adopted in combination with quantitative proteomics

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture, Fibroblast

SUBMITTER: Alfonsina D'Amato

LAB HEAD: Alfonsina D'Amato

PROVIDER: PXD031053 | Pride | 2022-02-22

REPOSITORIES: Pride

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Oxidative Stress Modulation by Carnosine in Scaffold Free Human Dermis Spheroids Model: A Proteomic Study.

Aiello Gilda G Rescigno Francesca F Meloni Marisa M Baron Giovanna G Aldini Giancarlo G Carini Marina M D'Amato Alfonsina A

International journal of molecular sciences 20220127 3

Carnosine is an endogenous β-alanyl-L-histidine dipeptide endowed with antioxidant and carbonyl scavenger properties, which is able to significantly prevent the visible signs of aging and photoaging. To investigate the mechanism of action of carnosine on human skin proteome, a 3D scaffold-free spheroid model of primary dermal fibroblasts from a 50-year-old donor was adopted in combination with quantitative proteomics for the first time. The label free proteomics approach based on high-resolution ...[more]

PMID: 35163388

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