Proteomics

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Differentiation of human induced pluripotent stem cells into induced motor neurons

ABSTRACT: Characterisation of induced motor neurons (iMN) generated from human induced pluripotent stem cells (hiPSC, hPSCreg name BIHi005-A) using doxycycline inducible expression of Ngn2, Isl1 and Lhx3. While this method of iMN generation has been described before (REF: doi.org/10.1002/cpcb.51), no extensive characterisation of the transcriptome and proteome throughout the differentiation process or of the mature neurons has been undertaken so far.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Pluripotent Stem Cell, Neuron, Motor Neuron

SUBMITTER: Tommaso Mari  

LAB HEAD: Matthias Selbach

PROVIDER: PXD031066 | Pride | 2025-11-24

REPOSITORIES: Pride

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Gonzo_20200422_TM_HS_MotoNeuron_Mn1.raw Raw
Gonzo_20200422_TM_HS_MotoNeuron_Mn10.raw Raw
Gonzo_20200422_TM_HS_MotoNeuron_Mn11.raw Raw
Gonzo_20200422_TM_HS_MotoNeuron_Mn12.raw Raw
Gonzo_20200422_TM_HS_MotoNeuron_Mn13.raw Raw
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Publications

Amyotrophic lateral sclerosis (ALS) is a neurodegenerative condition characterized by the progressive degeneration of motor neurons (MNs), ultimately resulting in death due to respiratory failure. A common feature among ALS cases is the early loss of axons, pointing to defects in axonal transport and translation as initial disease indicators. ALS is associated with mutations in RNA-binding proteins, such as FUS (Fused in Sarcoma). Here, we established a FUS<sup>R244RR</sup>-ALS hiPSC-derived mod  ...[more]

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