Discovery of Novel Circulating Immune Complexes in Lupus Nephritis Using Immunoproteomics

ABSTRACT: The goal is to discover novel circulating immune complexes (ICx) in the serum of Lupus Nephritis (LN) as potential biomarkers. Protein A/G magnetic beads or C1q-coated plates were used to capture ICx in the serum of LN, followed by the identification of immunoglobulin-binding proteins using liquid chromatography and tandem mass spectrometry (LC-MS/MS). Six related genomic-level databases were used for selecting potential biomarkers, following ELISA validation with independent samples.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Serum

DISEASE(S): Lupus Nephritis

SUBMITTER: chenling tang  

LAB HEAD: Tianfu Wu

PROVIDER: PXD031069 | Pride | 2022-05-20

REPOSITORIES: Pride