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To decipher an overall structure of a bacterial anti-plasmid defense system using cross-linking mass spectrometry.

ABSTRACT: The submitted data is from a BS3 crosslinked sample. The anti-plasmid defense system complex constitutes JetA, JetB, JetC, and JetD subunits. In this work, these four subunits were mixed to form a holo-subunit complex which was followed by crosslinking with isotopically/non-isotopically (1:1) mixture of BS3 reagent. The interactin subunit crosslinks were identified and this information was integrated with alphafold2 enabled models to construct an overall picture of a highly dynamic complex assembly

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Pseudomonas Aeruginosa Pa14

SUBMITTER: Amar Deep

LAB HEAD: Kevin D. Corbett

PROVIDER: PXD031096 | Pride | 2022-10-06

REPOSITORIES: Pride

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Bacteroidota assemble a unique type VI secretion system membrane complex

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