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Protein interactions with GFP-tagged cytoplasmic dynein-2 DATASET3

ABSTRACT: To identify those proteins interacting with the cytoplasmic dynein-2 using mammalian cell lines stably expressing GFP-tagged intermediate chain subunits, WDR34 (DYNC2I2) or WDR60 (DYNC2I1).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: David Stephens

LAB HEAD: David Stephens

PROVIDER: PXD031156 | Pride | 2023-01-24

REPOSITORIES: Pride

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Multiple interactions of the dynein-2 complex with the IFT-B complex are required for effective intraflagellar transport.

Hiyamizu Shunya S Qiu Hantian H Vuolo Laura L Stevenson Nicola L NL Shak Caroline C Heesom Kate J KJ Hamada Yuki Y Tsurumi Yuta Y Chiba Shuhei S Katoh Yohei Y Stephens David J DJ Nakayama Kazuhisa K

Journal of cell science 20230207 5

The dynein-2 complex must be transported anterogradely within cilia to then drive retrograde trafficking of the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. Here, we screened for potential interactions between the dynein-2 and IFT-B complexes and found multiple interactions among the dynein-2 and IFT-B subunits. In particular, WDR60 (also known as DYNC2I1) and the DYNC2H1-DYNC2LI1 dimer from dynein-2, and IFT54 (also known as TRAF3IP1) and IFT57 from IFT-B contr ...[more]

PMID: 36632779

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Protein interactions with GFP-tagged cytoplasmic dynein-2 DATASET2

Project description:To identify those proteins interacting with the cytoplasmic dynein-2 using mammalian cell lines stably expressing GFP-tagged intermediate chain subunits, WDR34 (DYNC2I2) or WDR60 (DYNC2I1).

2023-01-24 | PXD031158 | Pride

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Protein interactions with HA-tagged cytoplasmic dynein-2 DATASET1

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Protein interactions with HA-tagged WDR60 in CEP170 knockout cells

Project description:To identify those proteins interacting with the WDR60 subunit of cytoplasmic dynein-2 using mammalian cell lines stably expressing HA-tagged intermediate chain subunit WDR60 (DYNC2I1).

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Chromatin immunoprecipitation of Drosophila Schneider 2 cells using NELF-E and NELF-B antibodies

Project description:ChIP-chip analysis of GAGA factor and NELF in Drosophila S2 cells

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Coupling p53 binding and nucleosome occupancy measurements at p53 binding sites

Project description:We were interested to explain why p53 binds some high affinity sites in contrast to other high affinity sites that are not bound by p53. p53 binding was measured using p53 ChIP-CHIP and in parallel nucleosome occupancy was measured on these same sites Comparison between p53 binding and nucleosome occupancy at p53 predicted binding sites ChIP-CHIP of p53 from MCF7EcoR under Basal conditions and MCF7EcoR treated with NCS (Activated) and Mononucleosomal extraction from MCF7sip53, MCF7EcoR under Basal conditions and MCF7EcoR treated with NCS (Activated) Expression analysis of MCF7sip53 and MCF7EcoR treated with NCS (Activated)

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