Dataset Information

The secretome of Pseudoalteromonas prydzensis ACAM 620

ABSTRACT: Chitin-degrading enzymes secreted by Pseudoalteromonas prydzensis ACAM 620 were identified by proteomic analysis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Pseudoalteromonas Prydzensis Acam 620

SUBMITTER: Ping-Yi Li

LAB HEAD: Yuzhong Zhang

PROVIDER: PXD031176 | Pride | 2022-09-14

REPOSITORIES: Pride

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Publications

A pathway for chitin oxidation in marine bacteria.

Jiang Wen-Xin WX Li Ping-Yi PY Chen Xiu-Lan XL Zhang Yi-Shuo YS Wang Jing-Ping JP Wang Yan-Jun YJ Sheng Qi Q Sun Zhong-Zhi ZZ Qin Qi-Long QL Ren Xue-Bing XB Wang Peng P Song Xiao-Yan XY Chen Yin Y Zhang Yu-Zhong YZ

Nature communications 20221006 1

Oxidative degradation of chitin, initiated by lytic polysaccharide monooxygenases (LPMOs), contributes to microbial bioconversion of crystalline chitin, the second most abundant biopolymer in nature. However, our knowledge of oxidative chitin utilization pathways, beyond LPMOs, is very limited. Here, we describe a complete pathway for oxidative chitin degradation and its regulation in a marine bacterium, Pseudoalteromonas prydzensis. The pathway starts with LPMO-mediated extracellular breakdown ...[more]

PMID: 36202810

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Project description:Fomitiporia mediterranea (Fmed) is one of the main fungal species found in grapevine wood rot, also called “amadou”, one of the most typical symptoms of grapevine trunk disease Esca. This fungus is functionally classified as a white-rot, able to degrade all wood structure polymers, i.e., hemicelluloses, cellulose, and the most recalcitrant component, lignin. Specific enzymes are secreted by the fungus to degrade those components, namely carbohydrate active enzymes for hemicelluloses and cellulose, which can be highly specific for given polysaccharide, and peroxidases, which enable white-rot to degrade lignin, with specificities relating to lignin composition as well. Furthermore, besides polymers, a highly diverse set of metabolites often associated with antifungal activities is found in wood, this set differing among the various wood species. Wood decayers possess the ability to detoxify these specific extractives and this ability could reflect the adaptation of these fungi to their specific environment. The aim of this study is to better understand the molecular mechanisms used by Fmed to degrade wood structure, and in particular its potential adaptation to grapevine wood. To do so, Fmed was cultivated on sawdust from different origins: grapevine, beech, and spruce. Carbon mineralization rate, mass loss, wood structure polymers contents, targeted metabolites and secreted proteins were measured. We used the well-known white-rot model Trametes versicolor for comparison. Whereas no significant degradation was observed with spruce, a higher mass loss was measured on Fmed grapevine culture compared to beech culture. Moreover, on both substrates, a simultaneous degradation pattern and the degradation of wood extractives were demonstrated, and proteomic analyses identified a relative overproduction of oxidoreductases involved in lignin and extractive degradation on grapevine cultures, and only few differences in carbohydrate active enzymes. These results could explain at least partially the adaptation of Fmed to grapevine wood structural composition compared to other wood species and suggest that other biotic and abiotic factors should be considered to fully understand the potential adaptation of Fmed to its ecological niche.

Dataset Information

The secretome of Pseudoalteromonas prydzensis ACAM 620

Publications

A pathway for chitin oxidation in marine bacteria.

Similar Datasets

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