Proteomics

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Acetylome analysis of acetylation providing new insight into sclerotial generation in medicinal fungus Polyporus umbellatus


ABSTRACT: Polyporus umbellatus sclerotia are medicinal tissue, but little is known about acetylome of sclerotia generated from hyphae. Acetylated peptides in P. umbellatus were enriched by anti-acetyl-lysine antibody beaded agarose and DDA data were acquired by AB Triple TOF 5600+. Raw data were analyzed by ProteinPilot software to obtained acetylated peptides and proteins, and carried out bioinformatics analysis.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Polyporus Umbellatus Gsx09

TISSUE(S): Hypha

SUBMITTER: Bing Li  

LAB HEAD: Bing Li

PROVIDER: PXD031195 | Pride | 2022-05-20

REPOSITORIES: Pride

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Acetylome analysis of acetylation providing new insight into sclerotial generation in medicinal fungus Polyporus umbellatus.

Li Bing B   Liu Liu L   Shan Tingting T   Xing Yongmei Y   Guo Shunxing S  

Scientific reports 20220510 1


Sclerotium-forming fungi are ecologically diverse and possess notable pathogenic or medicinal properties. The sclerotial generation mechanism is still elusive though Polyporus umbellatus sclerotia are typical Traditional Chinese Medicine with diuretic and antitumor effects. Protein acetylation displays a crucial role in several biological processes, but the functions of acetylation in this valuable fungus are unknown at present. In this study, acetylome of P. umbellatus was studied using nano LC  ...[more]

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