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New insight into sclerotial generation in medicinal fungus Polyporus umbellatus

ABSTRACT: Polyporus umbellatus sclerotia are medicinal tissue, but little is known about acetylome of sclerotia generated from hyphae. Based on acetylome analysis results and expression levels of targeted protein that associated with sclerotia generation were verified by PRM using Orbitrap Fusion (Thermo-Fisher Scientific, San Jose, CA) mass spectrometer equipped with a nanospray Flex Ion Source and the UltiMate3000 RSLC nano (Dionex, Sunnyvale, CA).

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Polyporus Umbellatus Gsx09

SUBMITTER: Bing Li

LAB HEAD: Bing Li

PROVIDER: PXD031198 | Pride | 2022-05-20

REPOSITORIES: Pride

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Acetylome analysis of acetylation providing new insight into sclerotial generation in medicinal fungus Polyporus umbellatus.

Li Bing B Liu Liu L Shan Tingting T Xing Yongmei Y Guo Shunxing S

Scientific reports 20220510 1

Sclerotium-forming fungi are ecologically diverse and possess notable pathogenic or medicinal properties. The sclerotial generation mechanism is still elusive though Polyporus umbellatus sclerotia are typical Traditional Chinese Medicine with diuretic and antitumor effects. Protein acetylation displays a crucial role in several biological processes, but the functions of acetylation in this valuable fungus are unknown at present. In this study, acetylome of P. umbellatus was studied using nano LC ...[more]

PMID: 35538155

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