Name: Raw data for "Dynamic mitochondrial proteome under polyamines treatment in cardiac aging"
Keywords: Mitochondrial proteome

Dataset Information

Raw data for "Dynamic mitochondrial proteome under polyamines treatment in cardiac aging"

ABSTRACT: In this study, we found that spermine (Spm) and spermidine (Spd) injection for 6 weeks could prevent 24-month old rats heart dysfunction，improve mitochondrial function and down-regulate apoptosis. Using iTRAQ tools, we identify 75 mitochondrial proteins of statistically significant alteration in aging hearts, which mainly participate in important mitochondrial physiological activity, such as metabolism, translation, transport, apoptosis and oxidative phosphorylation.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Heart

SUBMITTER: Hao Zhang

LAB HEAD: Yajun Zhao

PROVIDER: PXD031270 | Pride | 2022-05-20

REPOSITORIES: Pride

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Publications

Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

Zhang Hao H Yan Meng M Liu Ting T Wei Peiling P Chai Nannan N Li Lingxu L Wang Junying J Yu Xue X Lin Yan Y Qiu Bintao B Zhao Yajun Y

Frontiers in cell and developmental biology 20220315

Age-related alteration of mitochondria causes impaired cardiac function, along with cellular and molecular changes. Polyamines can extend the life span in mice. However, whether polyamines can affect the dynamic mitochondrial proteome, thereby preventing age-related changes in cardiac function and cardiac aging, remains unclear. In this study, we found that spermine (Spm) and spermidine (Spd) injection for 6 weeks could prevent 24-month-old rats heart dysfunction, improve mitochondrial function, ...[more]

PMID: 35372351

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Project description:Erysipelothrix rhusiopathiae is a small, facultatively aerobic, gram-positive bacterium that causes erysipelas in swine and a wide spectrum of diseases in other animals, as well as the skin disease erysipeloid in humans. Swine erysipelas caused by E. rhusiopathiae is the most prevalent and economically important of these diseases. In China, swine erysipelas has emerged at an alarming rate, developing from scattered occurrences on a small number of farms to systemic outbreaks since 2012 in many provinces, thereby inflicting large financial losses. Some virulence factors of E. rhusiopathiae have been identified. The capsule is considered essential for resistance to phagocytosis and bacterial virulence. Neuraminidase plays a crucial role in initiating adhesion to endothelial cells. Two surface-adhesion proteins (RspA and RspB) participate in the initiation of biofilm formation by binding to abiotic and biotic surfaces. SpaA is involved in the adhesion of E. rhusiopathiae to porcine endothelial cells. Nevertheless, very little is known about the pathogenesis of E. rhusiopathiae. This knowledge gap has become one of the most important obstacles to controlling infection. Thus, the identification of novel virulence factors is necessary to improve understanding of the pathogenesis of E. rhusiopathiae.Surface proteins of gram-positive bacteria play a critical role in virulence by modifying the environment surrounding the cell. Furthermore, for many bacteria, surface proteins are candidate antigens for subunit vaccines. The examination of bacterial surface-protein fractions, including cell wall-associated proteins (CWPs), is an effective method for identifying novel virulence factors. One proteomic approach involves isobaric Tags for Relative and Absolute Quantitation (iTRAQ) coupled to liquid chromatography (LC)-quadrupole mass spectrometry (MS/MS). This method has been widely used for the comparative quantitative analysis of bacterial proteomes. In this study, iTRAQ combined with LC-MS/MS was applied to compare differentially abundant CWPs of high- and low-virulence strains of E. rhusiopathiae. Proteins with higher abundance in the high-virulence strain were found to aid in predicting potential virulence factors to improve vaccines or disease-control strategies.

Dataset Information

Raw data for "Dynamic mitochondrial proteome under polyamines treatment in cardiac aging"

Publications

Dynamic Mitochondrial Proteome Under Polyamines Treatment in Cardiac Aging.

Similar Datasets

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