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O Exploring dynamics of pre-germinative metabolism in Medicago truncatula seeds during priming, dry-back, and post-priming imbibition using proteomics

ABSTRACT: o The dramatic impact of climate change poses a demanding challenge: the need to enhance seed quality by designing tailored seed priming protocols. Proteomics can provide a comprehensive picture of the molecular players behind the seed response to priming. Using label-free protein quantification with mass spectrometry, changes in the seed proteome of the model legume Medicago truncatula were investigated along the rehydration-dehydration cycle of a standard vigorization treatment (hydropriming plus dry-back) and during post-priming imbibition.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Medicago Truncatula Var. Truncatula

TISSUE(S): Seed

SUBMITTER: Antje Dittmann

LAB HEAD: Alma Balestrazzi

PROVIDER: PXD031336 | Pride | 2023-07-10

REPOSITORIES: Pride

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Changes in <i>Medicago truncatula</i> seed proteome along the rehydration-dehydration cycle highlight new players in the genotoxic stress response.

Pagano Andrea A Kunz Laura L Dittmann Antje A Araújo Susana De Sousa SS Macovei Anca A Shridhar Gaonkar Shraddha S Sincinelli Federico F Wazeer Hisham H Balestrazzi Alma A

Frontiers in plant science 20230613

<h4>Introduction</h4>Several molecular aspects underlying the seed response to priming and the resulting vigor profile are still poorly understood. Mechanisms involved in genome maintenance deserve attention since the balance between stimulation of germination and DNA damage accumulation versus active repair is a key determinant for designing successful seed priming protocols.<h4>Methods</h4>Changes in the Medicago truncatula seed proteome were investigated in this study, using discovery mass sp ...[more]

PMID: 37409306

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