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Different roles of gamma- and beta-actin in the retina

ABSTRACT: Lack of cytoplasmic beta-actin gene (Actb) leads to early embryonic lethality in mice, however targeted editing of Actb coding sequence by introducing five point mutations to encode cytoplasmic gamma-actin protein does not affect mouse viability. These mice with beta to gamma actin replacement (Actbc-g mice) develop normally and show no detectable phenotypes at young age, despite complete lack of beta-actin protein. Here we investigated the effect of the replacement of beta-actin with gamma-actin in the retina.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Retina

SUBMITTER: Dawei Dong

LAB HEAD: Dawei W Dong

PROVIDER: PXD031465 | Pride | 2023-03-11

REPOSITORIES: Pride

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Differential N-terminal processing of beta and gamma actin.

Chen Li L Vedula Pavan P Tang Hsin Yao HY Dong Dawei W DW Kashina Anna S AS

iScience 20220923 10

Cytoplasmic beta- and gamma-actin are ubiquitously expressed in every eukaryotic cell. They are encoded by different genes, but their amino acid sequences differ only by four conservative substitutions at the N-termini, making it difficult to dissect their individual regulation. Here, we analyzed actin from cultured cells and tissues by mass spectrometry and found that beta, unlike gamma actin, undergoes sequential removal of N-terminal Asp residues, leading to truncated actin species found in b ...[more]

PMID: 36248738

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Different roles of gamma- and beta-actin in neuronal functions

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Active fluctuations of the nuclear envelope shape the transcriptional dynamics in oocytes

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