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40S peak fraction from human Expi293 cells

ABSTRACT: Mass spectrometry analysis of a sample derived from sucrose density gradient centrifugation of purified 80S ribosomes from human cells Expi293.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture

SUBMITTER: Simone Pellegrino

LAB HEAD: Alan John Warren

PROVIDER: PXD031525 | Pride | 2023-07-20

REPOSITORIES: Pride

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Cryo-EM reconstruction of the human 40S ribosomal subunit at 2.15 Å resolution.

Pellegrino Simone S Dent Kyle C KC Spikes Tobias T Warren Alan J AJ

Nucleic acids research 20230501 8

The chemical modification of ribosomal RNA and proteins is critical for ribosome assembly, for protein synthesis and may drive ribosome specialisation in development and disease. However, the inability to accurately visualise these modifications has limited mechanistic understanding of the role of these modifications in ribosome function. Here we report the 2.15 Å resolution cryo-EM reconstruction of the human 40S ribosomal subunit. We directly visualise post-transcriptional modifications within ...[more]

PMID: 36951107

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