Dataset Information

Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina, a Brazilian semiarid native woody plant

ABSTRACT: Erythrina velutina is widely used in traditional medicine, being a source of specialized metabolites with anti-inflammatory and central nervous system modulatory activities. To date, the specialized metabolic network of the genus Erythrina and its regulation are missing, in part due to the lack of genomic information. In an effort to fill this knowledge gap, leaves and seeds had their metabolic and proteomic profiles (LC/MS) analyzed and integrated with transcriptome (RNA-seq) data. These data sets were obtained from plants growing in their harsh natural habitat and examined with emphasis on differentially expressed transcripts, transcription factors, and genes putatively involved in the biosynthesis of flavonoids, the most abundant metabolite class annotated. Of the 96 metabolites distributed in different classes found in this study, 28 and 30 were exclusively found in seeds and leaves, respectively, whereas 38 metabolites occurred in both structures. It was possible to assemble a set of 17,822 transcripts, of which 3,937 transcripts were differentially expressed (2,815 down- and 1,122 up-regulated). Candidate transcripts involved in flavonoid accumulation and transcription factors amounted to 28 and 394, respectively. Expression patterns of transcripts involved in flavonoid biosynthesis and its regulation, as well as those involved in responses to biotic and abiotic stresses, are discussed in detail. Hopefully, these findings will provide a solid base to further the understanding of the molecular and metabolic responses in this important medicinal plant species, as well as identify key regulatory targets for future studies aiming at bioactive metabolite production.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Erythrina Velutina

TISSUE(S): Seed, Leaf

SUBMITTER: Daisy Chacon

LAB HEAD: Daisy Sotero Chacon

PROVIDER: PXD031557 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

Non-target molecular network and putative genes of flavonoid biosynthesis in <i>Erythrina velutina</i> Willd., a Brazilian semiarid native woody plant.

Chacon Daisy Sotero DS Santos Marlon Dias Mariano MDM Bonilauri Bernardo B Vilasboa Johnatan J da Costa Cibele Tesser CT da Silva Ivanice Bezerra IB Torres Taffarel de Melo TM de Araújo Thiago Ferreira TF Roque Alan de Araújo AA Pilon Alan Cesar AC Selegatto Denise Medeiros DM Freire Rafael Teixeira RT Reginaldo Fernanda Priscila Santos FPS Voigt Eduardo Luiz EL Zuanazzi José Angelo Silveira JAS Scortecci Kátia Castanho KC Cavalheiro Alberto José AJ Lopes Norberto Peporine NP Ferreira Leandro De Santis LS Dos Santos Leandro Vieira LV Fontes Wagner W de Sousa Marcelo Valle MV Carvalho Paulo Costa PC Fett-Neto Arthur Germano AG Giordani Raquel Brandt RB

Frontiers in plant science 20220908

<i>Erythrina velutina</i> is a Brazilian native tree of the Caatinga (a unique semiarid biome). It is widely used in traditional medicine showing anti-inflammatory and central nervous system modulating activities. The species is a rich source of specialized metabolites, mostly alkaloids and flavonoids. To date, genomic information, biosynthesis, and regulation of flavonoids remain unknown in this woody plant. As part of a larger ongoing research goal to better understand specialized metabolism i ...[more]

PMID: 36161018

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Dataset Information

Non-target molecular network and putative genes of flavonoid biosynthesis in Erythrina velutina, a Brazilian semiarid native woody plant

Publications

Non-target molecular network and putative genes of flavonoid biosynthesis in <i>Erythrina velutina</i> Willd., a Brazilian semiarid native woody plant.

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