Proteomics analysis of physiological effect of ZBTB16 (PLZF) on osteogenic differentiation of human valve interstitial cells (VICs) by DDA PASEF on TimsToF Pro

ABSTRACT: During fundamental proteotranscriptomic analysis of human valve interstitial cells (VICs) osteogenic differentiation, we revealed ZBTB16 (PLZF) as one of the most upregulated proteins. Thus, to investigate its physiological role in VICs osteogenic differentiation we performed its overexpression by lentiviral transduction with subsequent proteomics analysis. Despite obvious PLZF overexpression and its reinforcing effect on osteogenic differentiation, we revealed surprisingly small amount of differentially expressed proteins that assumed to be the targets of PLZF.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart Valve Cell

DISEASE(S): Aortic Valve Disease

SUBMITTER: Arseniy Lobov  

LAB HEAD: Anna B. Malashicheva

PROVIDER: PXD031572 | Pride | 2022-10-21

REPOSITORIES: Pride