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A universal and cost-effective method for general and specific RNA-protein complex isolation_18S rRNP capture

ABSTRACT: the establishment of a protocol isolating specific RNPs by combining a total RNP isolation with an antisense probe-mediated specific RNP isolation

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

TISSUE(S): Diploid Cell

SUBMITTER: Roosje Van Ende

LAB HEAD: Koen Geuten

PROVIDER: PXD031573 | Pride | 2023-05-10

REPOSITORIES: Pride

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A widely applicable and cost-effective method for specific RNA-protein complex isolation.

Balzarini Sam S Van Ende Roosje R Voet Arnout A Geuten Koen K

Scientific reports 20230427 1

Although methodological advances have been made over the past years, a widely applicable, easily scalable and cost-effective procedure that can be routinely used to isolate specific ribonucleoprotein complexes (RNPs) remains elusive. We describe the "Silica-based Acidic Phase Separation (SAPS)-capture" workflow. This versatile method combines previously described techniques in a cost-effective, optimal and widely applicable protocol. The specific RNP isolation procedure is performed on a pre-pur ...[more]

PMID: 37106019

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