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APEX2 labeling of Sec bodies in S2 cells

ABSTRACT: APEX2 was tagged to Sec24AB protein in S2 cells. Biotinylation was triggeredin resting cells and amino acid starved cells for comparative analyses of Sec24AB interactome. Sec24AB is localised to Sec bodies during starvation, thereby allowing the identification of protein components in Sec bodies.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Drosophila Melanogaster (fruit Fly)

TISSUE(S): Permanent Cell Line Cell

SUBMITTER: wei wu

LAB HEAD: Wei Wu

PROVIDER: PXD031601 | Pride | 2023-05-10

REPOSITORIES: Pride

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Novel Components of the Stress Assembly Sec Body Identified by Proximity Labeling.

Zhang Chujun C Kalaitsidou Elisavet E Damen J Mirjam A JMA Grond Rianne R Rabouille Catherine C Wu Wei W

Cells 20230330 7

Sec bodies are membraneless stress-induced assemblies that form by the coalescence of endoplasmic reticulum exit sites (ERES). Through APEX2 tagging of Sec24AB, we biotinylated and identified the full complement of Sec body proteins. In the presence of biotin-phenol and H2O2 (APEX on), APEX2 facilitates the transfer of a biotin moiety to nearby interactors of chimeric Sec24AB. Using this unbiased approach comparing APEX on and off (-H2O2) conditions, w ...[more]

PMID: 37048128

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