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LC-MS/MS Proteomics analysis of PDL1 protein in 293T cell line

ABSTRACT: To identify the proteins interact with PDL1 protein in human cell lines, we analyzed the protein complexes using tandem affinity purification followed by mass spectrometry (TAP-MS) in HEK293T. We established the cell lines stably expressing PDL1 gene fused with Flag tags. We identified proteins associated with the bait in the isolated complexes using MS and searched the human databases for these proteins.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Kidney

DISEASE(S): Breast Cancer

SUBMITTER: shaofang xie

LAB HEAD: Li Xu

PROVIDER: PXD031702 | Pride | 2022-10-13

REPOSITORIES: Pride

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