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HISTOLOGICAL AND PROTEOMIC CHARACTERIZATION OF MUSCULOSKELETAL AMYLOIDOMAS

ABSTRACT: We report the histological and immunohistochemical features of four cases of musculoskeletal amyloidoma in association with combined laser capture microdissection (LCM) of Congo Red (CR)-positive regions with a recent microproteomics workflow that improves the sensitivity of the analysis in order to confirm the nature of the protein deposit. Proteomics techniques allowed to elucidate the nature of the amyloid protein deposit, improving the results obtained by immunohistochemistry (IHC). IHC results were confirmed in two cases while laser-capture microdissection coupled with bottom-up microproteomics was necessary to type the other two cases, for which IHC was inconclusive.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Amyloid Plaque

SUBMITTER: Francesco Greco

LAB HEAD: Liam A. McDonnell

PROVIDER: PXD031789 | Pride | 2025-07-14

REPOSITORIES: Pride

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			Action	DRS
	Amyloidoma_Case_1.raw	Raw
	Amyloidoma_Case_2.raw	Raw
	Amyloidoma_Case_3.raw	Raw
	Amyloidoma_Case_4.raw	Raw
	Protein_list.xlsx	Xlsx

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Publications

Histological and proteomic characterization of musculoskeletal amyloidomas.

Gaeta Raffaele R Greco Francesco F Anastasi Federica F Andreani Lorenzo L Capanna Rodolfo R McDonnell Liam A LA Franchi Alessandro A

Pathologica 20250601 3

<h4>Introduction</h4>The term amyloidoma applies to localized deposits of amyloid in the absence of systemic amyloidosis. Skeletal and soft tissue amyloidomas are very rare and the pathogenesis is usually associated with lymphoproliferative disorders (plasmacytomas or plasmacytoid lymphomas) or as a consequence of local chronic inflammation.<h4>Methods</h4>In this paper we report the histological and immunohistochemical features of four cases of musculoskeletal amyloidoma in association with com ...[more]

PMID: 40626671

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