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Clathrin associated proteins in human malaria parasite Plasmodium falciparum

ABSTRACT: To reveal clathrin associated proteins in Plasmodium falciparum, clathrin heavy chain (CHC), clathrin light chain (CLC), and AP1 gamma subunit (AP1r) were tagged with GFP in the parasite. Immunoprecipitations (IPs) were performed with anti-GFP beads from parasite cytosol, parasitophorous vacuole (PV) and infected red blood cell membrane (iRBCM). clathrin coated vesicles (CCVs) were also purified from parasite by ultracentrifugation. Proteomic data from these pulldowns indicate that clathrin is majorly associated with AP1 subunits and involved in protein export. Surprisingly, we also discovered that clathrin is exported PV and deposited underneath the knobs in iRBCM.

INSTRUMENT(S):

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Single Nucleate Cell

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: JUN MIAO

LAB HEAD: Jun Miao

PROVIDER: PXD031805 | Pride | 2025-12-22

REPOSITORIES: Pride

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			Action	DRS
	20190118_Jun_clathrin-pull-down.raw	Raw
	20190118_Jun_clathrin-pull-down__F184434_.mzid.gz	Mzid
	20190118_Jun_clathrin-pull-down__F184434_.mzid_20190118_Jun_clathrin-pull-down__F184434_.MGF	Mzid
	20200810_3D7_M1.raw	Raw
	20200810_3D7_M1__F184435_.mzid.gz	Mzid

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