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Whole cell proteomics analysis of Trypanosoma brucei 75kDa invariant surface glycoprotein knockout cells

ABSTRACT: A superfamily of invariant surface glycoproteins (ISGs) populates the African trypanosome surface, one of which, ISG75, is implicated in uptake of the century-old drug suramin. We disrupted the ISG75 gene locus encoding an array of six ISG75 paralogs on chromosome V by CRISPR/Cas9 knockout. Here we characterise this knockout strain by quantitative whole cell proteomics. Additionally, we analyse the way ISG75 knockout cells respond to suramin on the proteome level.

INSTRUMENT(S): Q Exactive Plus, Q Exactive HF

ORGANISM(S): Trypanosoma Brucei

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Trypanosomiasis

SUBMITTER: Martin Zoltner

LAB HEAD: Mark C. Field

PROVIDER: PXD031887 | Pride | 2023-03-11

REPOSITORIES: Pride

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The role of invariant surface glycoprotein 75 in xenobiotic acquisition by African trypanosomes.

Makarov Alexandr A Began Jakub J Mautone Ileana Corvo IC Pinto Erika E Ferguson Liam L Zoltner Martin M Zoll Sebastian S Field Mark C MC

Microbial cell (Graz, Austria) 20230127 2

The surface proteins of parasitic protozoa mediate functions essential to survival within a host, including nutrient accumulation, environmental sensing and immune evasion. Several receptors involved in nutrient uptake and defence from the innate immune response have been described in African trypanosomes and, together with antigenic variation, contribute towards persistence within vertebrate hosts. Significantly, a superfamily of invariant surface glycoproteins (ISGs) populates the trypanosome ...[more]

PMID: 36789350

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