Whole cell proteomics analysis of Trypanosoma brucei 75kDa invariant surface glycoprotein knockout cells

ABSTRACT: A superfamily of invariant surface glycoproteins (ISGs) populates the African trypanosome surface, one of which, ISG75, is implicated in uptake of the century-old drug suramin. We disrupted the ISG75 gene locus encoding an array of six ISG75 paralogs on chromosome V by CRISPR/Cas9 knockout. Here we characterise this knockout strain by quantitative whole cell proteomics. Additionally, we analyse the way ISG75 knockout cells respond to suramin on the proteome level.

INSTRUMENT(S):

ORGANISM(S): Trypanosoma Brucei

TISSUE(S): Permanent Cell Line Cell

DISEASE(S): Trypanosomiasis

SUBMITTER: Martin Zoltner  

LAB HEAD: Mark C. Field

PROVIDER: PXD031887 | Pride | 2023-03-11

REPOSITORIES: Pride