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Comparison of in-solution and SP3 sample processing workflows on HeLa cell samples

ABSTRACT: Comparison of in-solution and single-pot, solid-phase-enchanced sample preparation (SP3) sample processing workflows on HeLa cell samples, using different lysis buffers based on SDS and guanidine hydrochloride, for optimal analysis by liquid chromatography mass spectrometry (LC-MS)

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma, Epithelial Cell, Cell Culture

SUBMITTER: Maria Hernandez-Valladares

LAB HEAD: Frode Berven

PROVIDER: PXD031918 | Pride | 2022-08-12

REPOSITORIES: Pride

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Comparing Efficiency of Lysis Buffer Solutions and Sample Preparation Methods for Liquid Chromatography-Mass Spectrometry Analysis of Human Cells and Plasma.

Neset Lasse L Takayidza Gracious G Berven Frode S FS Hernandez-Valladares Maria M

Molecules (Basel, Switzerland) 20220525 11

The use of a proper sample processing methodology for maximum proteome coverage and high-quality quantitative data is an important choice to make before initiating a liquid chromatography-mass spectrometry (LC-MS)-based proteomics study. Popular sample processing workflows for proteomics involve in-solution proteome digestion and single-pot, solid-phase-enhanced sample preparation (SP3). We tested them on both HeLa cells and human plasma samples, using lysis buffers containing SDS, or guanidiniu ...[more]

PMID: 35684327

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Three protein extraction methods for mass spectrometry proteomics of fresh frozen mouse tissue

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siRNA knockdown of Sp3 in human

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