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Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics

ABSTRACT: This project used a custom-built capillary electrophoresis (CE) mass spectrometry (MS) platform to demonstrate the scalable analysis of proteins from single cells of varying sizes and protein content in different vertebrate biological models. Neural tissue fated giant cells were identified in cleavage-stage Xenopus laevis (frog) embryos, and 18 ng from its protein content was analyzed. From cultured primary neurons from the mouse, diluted samples containing ~125 pg of protein digest was measured, estimating to a portion of the somal protein content. Compared to traditional nano-flow liquid chromatography (nanoLC) MS, CE-MS provided higher sensitivity and faster analysis. CE-ESI-HRMS offers a viable approach for scalable single-cell proteomics.

INSTRUMENT(S): Orbitrap Fusion Lumos, Q Exactive

ORGANISM(S): Xenopus Laevis (african Clawed Frog) Mus Musculus (mouse)

TISSUE(S): Brain, Embryo, Hippocampal Neuron, Early Embryonic Cell

SUBMITTER: Peter Nemes

LAB HEAD: Peter Nemes

PROVIDER: PXD031955 | Pride | 2022-03-02

REPOSITORIES: Pride

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Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics

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