Proteomics

Dataset Information

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Lung fibroblasts from chinese hamster cell line V79 and the effects of e-cigarettes liquids


ABSTRACT: Although still considered a safer alternative to classical cigarettes, growing body of work point to harmfull effects of e-cigarettes affecting range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use. In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluated by HPRT assay, genotoxicity by comet assay and the effect on cellular communication by metabolic cooperation assay. Additionally, comprehensive proteome analysis was performed via high resolution, parallel accumulation serial fragmentation-PASEF mass spectrometry. E-cigarette liquid concentration used in this study showed no mutagenic or genotoxic effect, however it negatively impacted metabolic cooperation between V79 cells. Both e-cigarette liquids induced significant depletion in total number of proteins and impairment of mitochondrial function in treated cells. The focal adhesion proteins were upregulated, which is in accordance with the results of metabolic cooperation assay. Increased presence of posttranslational modifications (PTMs), including carbonylations and direct oxidative modifications, was observed. Our study revealed impairment of metabolic cooperation as well as significant proteome and PTMs alterations in V79 cells treated with e-cigarette liquid warranting future studies on e- cigarettes health impact.

INSTRUMENT(S): autoflex

ORGANISM(S): Cricetulus Griseus (chinese Hamster) (cricetulus Barabensis Griseus)

TISSUE(S): Lung, Fibroblast

DISEASE(S): Chronic Obstructive Pulmonary Disease

SUBMITTER: Katarina Smiljanic  

LAB HEAD: Albert Sickmann

PROVIDER: PXD032071 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Electronic cigarette liquids impair metabolic cooperation and alter proteomic profiles in V79 cells.

Trifunovic Sara S   Smiljanić Katarina K   Sickmann Albert A   Solari Fiorella A FA   Kolarevic Stoimir S   Divac Rankov Aleksandra A   Ljujic Mila M  

Respiratory research 20220715 1


<h4>Background</h4>Although still considered a safer alternative to classical cigarettes, growing body of work points to harmful effects of electronic cigarettes (e-cigarettes) affecting a range of cellular processes. The biological effect of e-cigarettes needs to be investigated in more detail considering their widespread use.<h4>Methods</h4>In this study, we treated V79 lung fibroblasts with sub-cytotoxic concentration of e-cigarette liquids, with and without nicotine. Mutagenicity was evaluat  ...[more]

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