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Extracellular release of two peptidases dominates generation of the quorum sensing signal in Trypanosoma brucei

ABSTRACT: Trypanosomes causing African sleeping sickness use quorum sensing to control the production of transmission-competent stumpy forms in their mammalian hosts. This density-dependent process is signalled by the release of parasite peptidases whose action generates oligopeptides that stimulate the signal transduction pathway leading to stumpy formation. Using mass spectrometry analysis, we have characterised the peptidases released by Trypanosoma brucei brucei.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Trypanosoma Brucei

DISEASE(S): Trypanosomiasis

SUBMITTER: Keith Matthews

LAB HEAD: Professor Keith R. Matthews

PROVIDER: PXD032101 | Pride | 2022-08-12

REPOSITORIES: Pride

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Extracellular release of two peptidases dominates generation of the trypanosome quorum-sensing signal.

Tettey Mabel Deladem MD Rojas Federico F Matthews Keith R KR

Nature communications 20220609 1

Trypanosomes causing African sleeping sickness use quorum-sensing (QS) to generate transmission-competent stumpy forms in mammalian hosts. This density-dependent process is signalled by oligopeptides that stimulate the signal transduction pathway leading to stumpy formation. Here, using mass spectrometry analysis, we identify peptidases released by trypanosomes and, for 12 peptidases, confirm their extracellular delivery. Thereafter, we determine the contribution of each peptidase to QS signal p ...[more]

PMID: 35680928

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