Dataset Information

Exploration of the nurse shark (Ginglymostoma cirratum) plasma immunoproteome using high-throughput LC-MS/MS

ABSTRACT: LC-MS/MS proteomics was used to identify immune proteins in the plasma of the nurse shark (Ginglymostoma cirratum), using a de novo multi-tissue transcriptome generated for this species. LC-MS/MS was then used to assess the host response to immunization with human serum albumin (HSA) and Complete Freund’s Adjuvant (CFA).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Ginglymostoma Cirratum

TISSUE(S): Blood Plasma

SUBMITTER: David Stead

LAB HEAD: Dr Helen Dooley

PROVIDER: PXD032158 | Pride | 2022-08-12

REPOSITORIES: Pride

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Publications

Exploration of the Nurse Shark (<i>Ginglymostoma cirratum</i>) Plasma Immunoproteome Using High-Resolution LC-MS/MS.

Bakke Fiona K FK Gundappa Manu Kumar MK Matz Hanover H Stead David A DA Macqueen Daniel J DJ Dooley Helen H

Frontiers in immunology 20220606

Many animals of scientific importance lack species-specific reagents (e.g., monoclonal antibodies) for in-depth studies of immune proteins. Mass spectrometry (MS)-based proteomics has emerged as a useful method for monitoring changes in protein abundance and modifications in non-model species. It can be used to quantify hundreds of candidate immune molecules simultaneously without the generation of new reagents. Here, we used MS-based proteomics to identify and quantify candidate immune proteins ...[more]

PMID: 35734164

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Project description:Inducing the long-term protection via effector memory CD8+ T cells residing in the peripheral tissues is the ultimate goal of T cell-based vaccination strategies. CD8+ T cell reacting against a particular set of antigenic peptides show propensity to generate stable pools of memory inflating cells with profound protective capacity. While the epitopes that induce memory inflation have been thoroughly characterized and used as excellent constituents of vaccines such as recombinant adenoviruses, the identity of the tissue factors responsible for maintaining memory inflating CD8+ T cells remains elusive. Here, we have used a Cre recombinase-dependent, -galactosidase (gal)–recombinant adenovirus vector that allowed for cell-specific expression of gal epitopes and identification of cell types involved in generation of inflationary responses. While targeting antigen expression to classical hematopoietic antigen presenting cells was insufficient to activate gal-specific CD8+ T cells, expressing the antigen exclusively in CCL19-producing fibroblastic stromal cells (FSCs) induced robust anti-gal CD8+ T cell response. Using bone marrow chimera mice to abolish the expression of major histocompatibility complex I (MHC-I) and Basic Leucine Zipper ATF-Like Transcription Factor 3 (BATF3) in hematopoietic cells we demonstrated that CCL19-expressing FCS function as antigen libraries, gradually releasing the antigen to CD103+ DCs to maintain gal-specific memory inflating population. Moreover, targeted ablation of CCL19-producing cells revealed that pulmonary fibroblastic stromal cells act as the principal organizer of the nurturing niches that support the metabolic conversion in CD8+ T cells necessary for the generation of long-lasting protective inflationary responses. Overall, our data reveal a hitherto unknown function of CCL19-expressiong fibroblastic stromal in supporting the metabolic fitness of CD8+ T cells, thereby promoting the generation of tissue-specific and long-lasting protective CD8+ T cell responses.

Dataset Information

Exploration of the nurse shark (Ginglymostoma cirratum) plasma immunoproteome using high-throughput LC-MS/MS

Publications

Exploration of the Nurse Shark (<i>Ginglymostoma cirratum</i>) Plasma Immunoproteome Using High-Resolution LC-MS/MS.

Similar Datasets

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