Proteomic analysis response of rice (Oryza sativa) leaves to ultravoilet (UV-B) stress

ABSTRACT: Rice (Oryza staiva) is staple food for the human consumption and model plants for genetic and molecular studies. Only few studies have been conducted on ultraviolet-B (UV-B) stress on rice. UV-B stress effects different morphological and physiological changes in plants. However, underlying mechanisms governing these integrated responses are unknown. In this study, we conducted proteomic response of rice leaves to UV-B stress using two-dimensional gel electrophoresis and identified the selected proteins by mass spectrometry analysis. To identify changes in protein accumulation in response to UV-B stress, two cultivars, i.e., IR6 and REX were irradiated by artificial UV-B radiation. Four 4 levels of daily biologically effective UV-B radiation intensities, 0 (control), 5, 10, and 15 kJ m-2 d-1 were imposed. We conducted this experiment in Sunlit Soil-Plant-Atmosphere-Research (SPAR) chambers to mimic the natural environment.

INSTRUMENT(S): LTQ Orbitrap Velos Pro

ORGANISM(S): O. Sativa Indica Group Oryza Sativa Subsp. Japonica (rice)

TISSUE(S): Leaf

SUBMITTER: Saroj Sah  

LAB HEAD: Jiaxu Li

PROVIDER: PXD032163 | Pride | 2022-10-14

REPOSITORIES: Pride