Dataset Information

Systems-level nutrient stress analysis of soil grown Brassica napus shoots and roots by quantitative proteomics

ABSTRACT: Appropriate soil nutrient management is critical for modern Canola (Brassica napus L.) varieties and hybrids to meet their yield potentials. Canola fields are typically supplemented by the application of nitrogen, phosphorus, sulphur, and to a lesser extent, potassium fertilizers to maximize yield, while deficiency in these key macronutrients can result in severe growth phenotypes and significant yield losses. To date, our understanding of canola nutrient deficiency responses is disparate, encompassing a large number of varieties using a variety of technologies and assessment criteria, with seminal understanding of the overlaps between nutrient deficiencies responses, having not yet been defined, placing limitations on our ability to increase the nutrient efficiency of this critical oil seed. To address this, we performed a comparative quantitative proteomics analysis of both shoot and root tissue harvested from soil-grown Canola plants experiencing either nitrogen, phosphorus, potassium, or sulphur deficiency. Our results show intriguing similarities in plant responses to deficiency in multiple nutrients. We also find very distinct proteome-level changes between shoot and root tissue of plants experiencing nutrient stress, suggesting the presence of highly organ-specific responses to nutrient deficiency. Our results pave the way for a more comprehensive understanding of the shared and distinct response mechanisms of plants to multiple essential nutrients.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Brassica Napus (rape)

TISSUE(S): Plant Cell, Root, Shoot

SUBMITTER: Richard Uhrig

LAB HEAD: Richard Glen Uhrig

PROVIDER: PXD032223 | Pride | 2025-05-06

REPOSITORIES: Pride

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Quantitative Proteomic Analysis of Brassica Napus Reveals Intersections Between Nutrient Deficiency Responses.

Grubb L E LE Scandola S S Mehta D D Khodabocus I I Uhrig R G RG

Plant, cell & environment 20241024 2

Macronutrients such as nitrogen (N), phosphorus (P), potassium (K) and sulphur (S) are critical for plant growth and development. Field-grown canola (Brassica napus L.) is supplemented with fertilizers to maximize plant productivity, while deficiency in these nutrients can cause significant yield loss. A holistic understanding of the interplay between these nutrient deficiency responses in a single study and canola cultivar is thus far lacking, hindering efforts to increase the nutrient use effi ...[more]

PMID: 39449274

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Project description:Clubroot of Brassicaceae, an economically important soil borne disease, is caused by Plasmodiophora brassicae Woronin, an obligate, biotrophic protist. This disease poses a serious threat to canola and related crops in Canada and around the globe causing significant loss to seed yield. The pathogen is continuously evolving and new pathotypes are emerging, this necessitates the development of novel resistant canola cultivars to manage the disease effectively. Given that proteins play a crucial role in majority of biological processes and molecular functions, the identification of differentially abundant proteins (DAP) using proteomics information is an attractive approach to understand the plant-pathogen interactions as well as in the future development of gene specific markers for developing clubroot resistant (CR) cultivars. In this study, P. brassicae pathotype 3 (P3H) was used to challenge CR and clubroot susceptible (CS) canola lines. Root samples were collected at three distinct stages of pathogenesis, 7-, 14-, and 21-days post inoculation (DPI), protein samples were isolated, digested with trypsin and subjected to LC-MS/MS analysis. A total of 937 proteins demonstrated a significant (q < 0.05) change in abundance in at least in one of the time points when compared between control and inoculated CR-parent, CR-progeny, CS-parent, CS-progeny and 784 proteins were significantly (q < 0.05) changed in abundance in at least in one of the time points when compared between the inoculated- CR and CS root proteomes of parent and progeny across the three time points tested. Functional annotation of the differentially abundant proteins (DAPs) revealed several proteins related to calcium dependent signaling pathways in response to the pathogen. In addition, proteins related to reactive oxygen species (ROS) biochemistry, dehydrins, lignin, thaumatin, and phytohormones were identified. Among the DAPs, 74 putative proteins orthologous to CR proteins and quantitative trait loci (QTL) associated with eight CR loci in four chromosomes including chromosomes A3 and A8 were identified. In conclusion, these results have contributed to an improved understanding of the mechanisms that are involved in mediating response to P. brassicae in canola at the protein level.

			Action	DRS
	20201001_BN_19_TP_K2.raw	Raw
	20201001_BN_20_TP_P2.raw	Raw
	20201001_BN_21_TP_C4.raw	Raw
	20201001_BN_22_TP_P1.raw	Raw
	20201001_BN_23_TP_N3.raw	Raw