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TMT-labeled mass spectrometry-based proteomics analysis in NCI-H1581 lung cancer cells upon AZD4547 treatment

ABSTRACT: FGFR1-amplified lung cancer cell line NCI-H1581 was treated with FGFR inhibitor AZD4547 (0.1μM) or DMSO vehicle for 24 hours. Then TMT-labeled mass spectrometry-based proteomics was carried out in cell lysates to analyze the differentially expressed proteins between two groups.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Epithelial Cell Of Lung

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Jing Gao

LAB HEAD: Zhou Hu

PROVIDER: PXD032227 | Pride | 2022-10-13

REPOSITORIES: Pride

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Publications

18F-FDG PET as an imaging biomarker for the response to FGFR-targeted therapy of cancer cells via FGFR-initiated mTOR/HK2 axis.

Jiang Yuchen Y Zeng Qinghe Q Jiang Qinghui Q Peng Xia X Gao Jing J Wan Haiyan H Wang Luting L Gao Yinglei Y Zhou Xiaoyu X Lin Dongze D Feng Hanyi H Liang Sheng S Zhou Hu H Ding Jian J Ai Jing J Huang Ruimin R

Theranostics 20220829 14

Rationale: The overall clinical response to FGFR inhibitor (FGFRi) is far from satisfactory in cancer patients stratified by FGFR aberration, the current biomarker in clinical practice. A novel biomarker to evaluate the therapeutic response to FGFRi in a non-invasive and dynamic manner is thus greatly desired. Methods: Six FGFR-aberrant cancer cell lines were used, including four FGFRi-sensitive ones (NCI-H1581, NCI-H716, RT112 and Hep3B) and two FGFRi-resistant ones (primary for N ...[more]

PMID: 36168616

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