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Profiling Glutathionylome in CD38-mediated Epithelial-Mesenchymal Transition

ABSTRACT: We engineered a cell system in which the glutathione synthetase (GS) mutant was expressed that catalyzed the formation of a glutathione analogy from azido-alanine to profile changes of glutathionylome in CD38-overexpressing cells.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Non-small Cell Lung Carcinoma

SUBMITTER: Ma Yingying

LAB HEAD: Deng, Haiteng

PROVIDER: PXD032233 | Pride | 2022-05-31

REPOSITORIES: Pride

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Profiling Glutathionylome in CD38-Mediated Epithelial-Mesenchymal Transition.

Ma Yingying Y Zhu Songbiao S Yi Meiqi M Zhang Wenhao W Xue Yuanyuan Y Liu Xiaohui X Deng Haiteng H

Journal of proteome research 20220414 5

Protein S-glutathionylation is an important posttranslational modification that regulates various cellular processes. However, changes in glutathionylome in epithelial-mesenchymal transition (EMT), a crucial cellular process for embryonic development, wound healing, and carcinoma progression and metastasis, have not been fully characterized. Our previous study revealed that CD38 overexpression decreased cellular nicotinamide adenine dinucleotide (NAD<sup>+</sup>) levels and caused cells to under ...[more]

PMID: 35420434

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