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C. elegans phosphoproteomics - DAF15 knockdown (The TORC1 phosphoproteome in C. elegans reveals roles in transcription and autophagy)

ABSTRACT: The protein kinase complex Target of Rapamycin complex 1 (TORC1) is a critical mediator of nutrient sensing that has been widely studied in cultured cells and yeast, yet our understanding of the regulatory activities of TORC1 in the context of a whole, multi-cellular organism is still very limited. Using C. elegans, we analyzed the DAF-15/Raptor-dependent phosphoproteome by quantitative mass spectrometry and characterized direct kinase targets by in vitro kinase assays. Here we show new targets of TORC1 that indicate previously unknown regulation of transcription and autophagy. Our results further show that DAF-15/Raptor is differentially expressed during postembryonic development, suggesting a dynamic role for TORC1 signaling throughout development. This study provides a comprehensive view of the TORC1 phosphoproteome and reveals more than 100 DAF-15/Raptor-dependent phosphosites, reflecting the complex function of TORC1 in a whole, multi-cellular organism.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Zach Poss

LAB HEAD: Min Han

PROVIDER: PXD032260 | Pride | 2022-04-22

REPOSITORIES: Pride

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