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Monoclonal antibody NIST8671 LC-MS/MS

ABSTRACT: The characteristics of monoclonal antibodies (mAbs) cohering various function effectors shows great expectation in therapy. Glycosylation, one of the common post-translational modifications, deeply influences the cohesion. It is necessary to grasp monosaccharide composition/sequence and glycan structures in mAbs. There have been comprehensive mass spectrometry characterization of N-glycosylation of mAbs, and monosaccharide compositions are deduced according to known biosynthetic rules. Our recently developed intact N-glycopeptide search engine GPSeeker has made unambiuous structure-specific characterization of N-glycosylation possible with structure-diagnostic fragment ions from selective fragmentattion of N-glycan moieties.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

SUBMITTER: Bi Ming

LAB HEAD: Zhixin Tian

PROVIDER: PXD032275 | Pride | 2022-04-14

REPOSITORIES: Pride

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Structure-Specific <i>N-</i>Glycoproteomics Characterization of NIST Monoclonal Antibody Reference Material 8671.

Bi Ming M Bai Bing B Tian Zhixin Z

Journal of proteome research 20220329 5

The characteristics of monoclonal antibodies (mAbs) cohering various function effectors show great expectation in therapy. Glycosylation, one of the common post-translational modifications, deeply influences cohesion. It is necessary to grasp monosaccharide composition/sequence and glycan structures in mAbs. There has been comprehensive mass spectrometry characterization of <i>N-</i>glycosylation of mAbs, and monosaccharide compositions are deduced according to known biosynthetic rules. Our rece ...[more]

PMID: 35349291

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