Pattern Analysis of Organellar Maps for Interpretation of Pro-teomic Data

ABSTRACT: The human proton-coupled folate transporter (PCFT) was stably expressed as a fu-sion protein with a BioID2-HA in the C-terminal position (PCFT-BioID2). The uncom-plxed biotin transferase was used as a control to identify proteins that are biotinylated randomly due to sheer abundance and/or an unnatural affinity to the BioID2 ligase. HeLa PCFT-BioID2 cells expressed the PCFT fusion protein which localized to the plasma mem-brane and exhibited robust transport typical of PCFT. HeLa PCFT-BioID2 and BioID2 cells were cultured in complete DMEM (biotin-free), with and without biotin (50 μM; 16 h) then biotinylated proteins were selected on streptavidin beads.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Hela Cell

SUBMITTER: Jordan Burton  

LAB HEAD: Paul M. Stemmer

PROVIDER: PXD032297 | Pride | 2022-06-09

REPOSITORIES: Pride