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MS-based proteomic characterization of Toxoplasma gondii GRA28-associated complexes

ABSTRACT: The obligate intracellular protozoan Toxoplasma gondii exploits the trafficking of mononuclear phagocytes for systemic dissemination. We report that, upon T. gondii infection, macrophages initiate expression of transcription factors normally attributed to DCs, upregulate the expression of Ccr7 with a chemotactic response, and perform systemic migration when adoptively transferred into mice. We identify the parasite effector GRA28, which is secreted into the host cell nuclei, as driving the chemotactic migratory activation of parasitized macrophages. To gain insight about the molecular role played by GRA28 in this phenomenon, we characterized its interactome.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii Rh Mus Musculus (mouse)

TISSUE(S): Macrophage

SUBMITTER: Yohann Couté

LAB HEAD: Yohann Couté

PROVIDER: PXD032360 | Pride | 2022-11-02

REPOSITORIES: Pride

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The Toxoplasma effector GRA28 promotes parasite dissemination by inducing dendritic cell-like migratory properties in infected macrophages.

Ten Hoeve Arne L AL Braun Laurence L Rodriguez Matias E ME Olivera Gabriela C GC Bougdour Alexandre A Belmudes Lucid L Couté Yohann Y Saeij Jeroen P J JPJ Hakimi Mohamed-Ali MA Barragan Antonio A

Cell host & microbe 20221028 11

Upon pathogen detection, macrophages normally stay sessile in tissues while dendritic cells (DCs) migrate to secondary lymphoid tissues. The obligate intracellular protozoan Toxoplasma gondii exploits the trafficking of mononuclear phagocytes for dissemination via unclear mechanisms. We report that, upon T. gondii infection, macrophages initiate the expression of transcription factors normally attributed to DCs, upregulate CCR7 expression with a chemotactic response, and perform systemic migrati ...[more]

PMID: 36309013

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