Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metaproteomic profiling of the intestinal microbiota of the participants of the AGBRESA study

ABSTRACT: Application of a mass spectrometry -based approach to assess the gut microbiota composition and the associated metaproteomic functionality of subjects exposed to simulated microgravityduring the Artificial Gravity Bed Rest study (AGBRESA)

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Archaea Bacteria Viruses Eukaryota (eucaryotes)

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD032387 | Pride | 2023-10-24

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Publications

Human gut microbiome and metabolite dynamics under simulated microgravity.

Ramos-Nascimento Ana A Grenga Lucia L Haange Sven-Bastiaan SB Himmelmann Alexandra A Arndt Franca Sabine FS Ly Yen-Tran YT Miotello Guylaine G Pible Olivier O Jehmlich Nico N Engelmann Beatrice B von Bergen Martin M Mulder Edwin E Frings-Meuthen Petra P Hellweg Christine Elisabeth CE Jordan Jens J Rolle-Kampczyk Ulrike U Armengaud Jean J Moeller Ralf R

Gut microbes 20230925 2

The Artificial Gravity Bed Rest - European Space Agency (AGBRESA) study was the first joint bed rest study by ESA, DLR, and NASA that examined the effect of simulated weightlessness on the human body and assessed the potential benefits of artificial gravity as a countermeasure in an analog of long-duration spaceflight. In this study, we investigated the impact of simulated microgravity on the gut microbiome of 12 participants during a 60-day head-down tilt bed rest at the :envihab facilities. Ov ...[more]

PMID: 37749878

Similar Datasets

Metaproteomic profiling of the intestinal microbiota of SARS-CoV-2 infected patients

Project description:Application of a mass spectrometry -based approach to assess the gut microbiota composition and the associated metaproteomic functionality in patients with intestinal COVID-19 infection

2022-09-01 | PXD024990 | Pride

Estimation of relative biomasses in microbiota

Project description:Presentation of a new methodology to assess microbiota relative quantifications in metaproteomics

2020-05-27 | PXD015500 | Pride

Metaproteomic profiling of induced and spontaneous sputum samples from cystic fibrosis patients

Project description:Application of a mass spectrometry -based approach to assess the lung microbiota composition and the associated metaproteomic functionality of subjects with cystic fibrosis

2023-02-04 | PXD034628 | Pride

Analysis of H. kunzii H13 under conditions that mimic the wound environment

Project description:Analysis of exoproteome and proteome of H. kunzii H13 at exponential and stationary phase of growth

2023-05-21 | PXD034903 | Pride

Metaproteomics on the digestive tract from Gammarus fossarum, an environmentally relevant sentinel.

Project description:Presentation of a new data treatment methodology for investigating the full range of biomasses and metabolic activities of the holobiont components from the digestive tract of Gammarus fossarum. Two tissues, intestine and hepatopancreatic caecum from individual animals were compared. Three animals have been analyzed in technical duplicates.

2021-09-09 | PXD016248 | Pride

Effects of a nutritional supplement in older individuals subject to ten days of complete bed rest.

Project description:Older individuals subjected to complete bed rest for 10 days were randomized to a complete nutritional supplement with or without hydroxymethyl butyrate (HMB). Muscle biopsies from the vastus lateralis were obtained immediately prior and at the conclusion of the bed rest. RNA-sequencing was performed to determine the gene expression changes associated with bed rest. Marked decrease in the expression of genes associated with mitochondrial energy metabolism including fatty acid oxidation, TCA cycle and the electron transport chain was observed with bed rest. These effects were partly mitigated in the subjects provided the nutritional supplement with HMB.

2019-11-01 | GSE130722 | GEO

Insulin resistance induced by physical inactivity is associated with multiple transcriptional changes in skeletal muscle in young men

Project description:Rationale: Physical inactivity is a risk factor for insulin resistance. We examined the effect of nine days of bed rest on basal and insulin stimulated expression of genes potentially involved in insulin action by applying hypothesis-generating microarray in parallel with candidate gene real-time PCR approaches in 20 healthy, young men. Furthermore, we investigated whether bed rest affected DNA methylation in the promoter region of the peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PPARGC1A) gene. Subjects were re-examined after four weeks of retraining. Findings: Bed rest induced insulin resistance and altered the expression of more than 4,500 genes. These changes were only partly normalized after four weeks of retraining. Pathway analyses revealed significant down-regulation of 34 pathways, predominantly those of genes associated with mitochondrial function including PPARGC1A. Despite induction of insulin resistance, bed rest resulted in a paradoxically increased response to acute insulin in the general expression of genes, particularly those involved in inflammation and endoplasmatic reticulum (ER) stress. Furthermore, bed rest changed gene expressions of several insulin resistance and diabetes candidate genes. We also observed a trend toward increased PPARGC1A DNA methylation after bed rest. Conclusions: Impaired expression of PPARGC1A and other genes involved in mitochondrial function as well as a paradoxically increased response to insulin of genes involved in inflammation and ER stress may contribute to the development of insulin resistance induced by bed rest. Lack of complete normalization of changes after four weeks of exercise retraining underscores the importance of maintaining a minimum of daily physical activity.

2010-09-21 | GSE24215 | GEO

Gene expression in skeletal muscle in older individuals subject to ten days of complete bed rest.

Project description:Older individuals were subjected to complete bed rest for 10 days. Muscle biopsies from the vastus lateralis were obtained immediately prior and at the conclusion of the bed rest. RNA-sequencing was performed to determine the gene expression changes associated with bed rest. Marked decrease in the expression of genes associated with mitochondrial energy metabolism including fatty acid oxidation, TCA cycle and the electron transport chain was observed. Upstream regulator analysis revealed that many of these genes are downstream targets of the transcription factors PPAR, ERR and PGC-1alpha.

2019-09-24 | GSE126865 | GEO

Insulin resistance induced by physical inactivity is associated with multiple transcriptional changes in skeletal muscle in young men

2010-09-21 | E-GEOD-24215 | biostudies-arrayexpress

Human (21d bed rest) Muscle LC-MSMS

Project description:Muscle atrophy is one of the main deleterious consequences of physical inactivity. Bed rest represents a unique model of physical inactivity and results in main changes in muscle metabolic function. However, our knowledge on the detrimental effects of bed rest still needs clarification. The aim of this study was to decipher the mechanisms involved in disuse muscle atrophy and the effects of different countermeasures in healthy men. A cross-over trial was designed, with each trial consisting in a 7-day ambulatory control period for baseline data collection, followed by 21 days head-down tilt bed rest with or without resistive vibration exercise only or combined with nutritional (protein) supplement as countermeasures. The changes in the Vastus lateralis muscle proteome collected in the eight volunteers were characterized using mass spectrometry-based label-free quantitative proteomics.

2020-07-07 | PXD006882 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data