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Comparison of performances of automatic versus manual liquid handling in density-based separation of extracellular vesicles from plasma and urine

ABSTRACT: Extracellular vesicles (EV) in body fluids are extensively studied as potential biomarkers for numerous diseases. Major impediments of EV-based biomarker discovery include the manual labor, and the specificity and reproducibility of EV sample preparation. To tackle this, we present an automated liquid handling workstation for the density-based separation of EV from human body fluids and compare its performance to manual handling by (in)experienced researchers. To validate automated density-based separation of EVs from human body fluids, including blood plasma and urine, we assess variation, yield and purity by mass spectrometry-based proteomics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Urine, Blood Plasma

SUBMITTER: Pekka Rappu

LAB HEAD: Olivier De Wever

PROVIDER: PXD032394 | Pride | 2023-05-10

REPOSITORIES: Pride

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