Analysis of subcellular Importin b1 interactomes by BioID and BAR proximity labelling

ABSTRACT: Importin 1 (KPNB1) is a nucleocytoplasmic transport factor with critical roles in both cytoplasmic and nucleocytoplasmic transport, hence there is keen interest in the characterization of its subcellular interactomes. We found limited efficiency of BioID in detection of importin complex cargos, and therefore generated a highly specific and sensitive anti-KPNB1 monoclonal antibody to enable Biotinylation by Antibody Recognition (BAR) analysis of Importin 1 interactomes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Spinal Cord Motor Neuron, Hek-293 Cell, Hela Cell

SUBMITTER: Juan Oses-Prieto  

LAB HEAD: Mike Fainzilber

PROVIDER: PXD032728 | Pride | 2022-10-15

REPOSITORIES: Pride