PXD032757 - The role of extracellular vesicles in synovial fibroblast senescence

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The role of extracellular vesicles in synovial fibroblast senescence

ABSTRACT: In arthritis, synovial fibroblast (SF) senescence is linked to the activation of a pro-inflammatory phenotype contributing to chronic arthritis pathogenesis. Additionally, senescent cells accumulate in ageing tissues further promoting ageing and inflammation. These cells have dysregulated mitochondrial function and metabolism, limited tissue regeneration, produce reactive oxygen species (ROS) and secrete bioactive molecules, including pro-inflammatory cytokines, chemokines and matrix-remodelling enzymes known as SASP (senescence-associated secretory phenotype). In vitro, senescent cells can induce a senescent phenotype in surrounding bystander cells. Interestingly, extracellular vesicles (EVs) have critical roles in cellular senescence and ageing and are mediators in intercellular communication. We hypothesize that senescent cells in osteoarthritic SFs induce senescence and/or a proinflammatory phenotype in non-senescent osteoarthritic SFs, mediated through EV cargo.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: James Anderson

LAB HEAD: Mandy Jayne Peffers

PROVIDER: PXD032757 | Pride | 2022-10-15

REPOSITORIES: Pride

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Publications

The role of extracellular vesicle miRNAs and tRNAs in synovial fibroblast senescence.

Wijesinghe Susanne N SN Anderson James J Brown Thomas J TJ Nanus Dominika E DE Housmans Bas B Green Jonathan A JA Hackl Matthias M Choi Katie K KK Arkill Kenton P KP Welting Tim T James Victoria V Jones Simon W SW Peffers Mandy J MJ

Frontiers in molecular biosciences 20220923

Extracellular vesicles are mediators of intercellular communication with critical roles in cellular senescence and ageing. In arthritis, senescence is linked to the activation of a pro-inflammatory phenotype contributing to chronic arthritis pathogenesis. We hypothesised that senescent osteoarthritic synovial fibroblasts induce senescence and a pro-inflammatory phenotype in non-senescent osteoarthritic fibroblasts, mediated through extracellular vesicle cargo. Small RNA-sequencing and mass spect ...[more]

PMID: 36213127

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