Name: IP mass spectrometry of CRY2 in dark and blue light
Keywords: Cry2 blue light

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IP mass spectrometry of CRY2 in dark and blue light

ABSTRACT: IP mass spectrometry of Arabidopsis CRY2 in dark and blue light

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Seedling

SUBMITTER: Li Zhang

LAB HEAD: zecheng Zuo

PROVIDER: PXD032848 | Pride | 2022-06-09

REPOSITORIES: Pride

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Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clock.

Mo Weiliang W Zhang Junchuan J Zhang Li L Yang Zhenming Z Yang Liang L Yao Nan N Xiao Yong Y Li Tianhong T Li Yaxing Y Zhang Guangmei G Bian Mingdi M Du Xinglin X Zuo Zecheng Z

Nature communications 20220512 1

Cryptochromes are blue light receptors that regulate plant growth and development. They also act as the core components of the central clock oscillator in animals. Although plant cryptochromes have been reported to regulate the circadian clock in blue light, how they do so is unclear. Here we show that Arabidopsis cryptochrome 2 (CRY2) forms photobodies with the TCP22 transcription factor in response to blue light in plant cells. We provide evidence that PPK kinases influence the characteristics ...[more]

PMID: 35551190

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Cryptochromes interact directly with PIFs to control plant growth in limiting blue light

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UBP12 and UBP13 deubiquitinases destabilize the CRY2 blue light receptor to regulate Arabidopsis growth

Project description:Light is a crucial exogenous signal sensed by cryptochrome (CRY) blue light receptors to modulate growth and the circadian clock in plants and animals. However, how CRYs interpret light quantity to regulate growth in plants remains poorly understood. Furthermore, CRY2 protein levels and activity are tightly regulated in light to fine-tune hypocotyl growth; however, details of the mechanisms that explain precise control of CRY2 levels are not fully understood. We show that in Arabidopsis, UBP12 and UBP13 deubiquitinases physically interact with CRY2 in light. UBP12/13 negatively regulates CRY2 by promoting its ubiquitination and turnover to modulate hypocotyl growth. Growth and development were explicitly affected in blue light when UBP12/13 were disrupted or overexpressed, indicating their role alongside CRY2. UBP12/13 also interacted with and stabilized COP1, which is partially required for CRY2 turnover. Our combined genetic and molecular data support a mechanistic model in which UBP12/13 interact with CRY2 and COP1, leading to the stabilization of COP1. Stabilized COP1 then promotes the ubiquitination and degradation of CRY2 under blue light. Despite decades of studies on deubiquitinases, the knowledge of how their activity is regulated is limited. Our study provides insight into how exogenous signals and ligands, along with their receptors, regulate deubiquitinase activity by protein-protein interaction. Collectively, our results provide a framework of cryptochromes and deubiquitinases to detect and interpret light signals to control plant growth at the most appropriate time.

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