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DNA methyltransferase (DNMT) associated proteins in human malaria parasite Plasmodium falciparum

ABSTRACT: This study identified Plasmodium falciparum DNA methyltransferase (DNMT)associated proteins in parasite cytosol and nucleus, including RNA binding proteins and AP2-domain containing proteins, indicating this protein is involved in RNA process and transctiptional regulation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Single Nucleate Cell

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: JUN MIAO

LAB HEAD: Jun Miao

PROVIDER: PXD032860 | Pride | 2023-07-20

REPOSITORIES: Pride

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Characterization of the dual role of Plasmodium falciparum DNA methyltransferase in regulating transcription and translation.

Lucky Amuza B AB Wang Chengqi C Li Xiaolian X Chim-Ong Anongruk A Adapa Swamy R SR Quinlivan Eoin P EP Jiang Rays R Cui Liwang L Miao Jun J

Nucleic acids research 20230501 8

DNA modifications are critical in fine-tuning the biological processes in model organisms. However, the presence of cytosine methylation (5mC) and the function of the putative DNA methyltransferase, PfDNMT2, in the human malaria pathogen, Plasmodium falciparum, remain controversial. Here, we revisited the 5mC in the parasite genome and the function of PfDNMT2. Low levels of genomic 5mC (0.1-0.2%) during asexual development were identified using a sensitive mass spectrometry procedure. Native PfD ...[more]

PMID: 37026483

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