Combining recombinase-mediated cassette exchange strategy with quantitative proteomic and phosphoproteomic analyses to inspect intracellular functions of the tumor supressor galectin-4 in colorectal cancer cells.

ABSTRACT: In order to systematically explore Galectin-4 function in CRC we established a CRC cell line where Gal4 expression can be regulated via doxycycline (dox)-inducible expression of a single copy wildtype LGALS4 transgene generated by recombinase-mediated cassette exchange (RMCE). Using this model and applying in depth proteomic and phosphoproteomic analyses we systematically screened for intracellular changes induced by Gal4 expression by.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Malwina Michalak  

LAB HEAD: Juergen Kopitz

PROVIDER: PXD032902 | Pride | 2022-08-12

REPOSITORIES: Pride