Characterization of pathogenic Candida glabrata and their effectiveness against systemic candidiasis in BALB/c mice

ABSTRACT: Candida glabrata is a common opportunistic fungal pathogen of humans, with the emergence of resitant strains being of particulary concern. The cell wall associated and secretory proteins are intrinsically involved in vital functions during the fungal-host interactions. These proteins are responsible for eliciting immune response in host during candidiasis and therefore can be a promising candidates for vaccine development. BALB/c mice were broadly divided into unimmunized, immunized groups and the designated groups were immunized with β-mercaptoethanol (β-ME) call wall extract, and secretory protein extract from C. glabrata via intraperitoneal route. All the animals were then challenged with a lethal dose of C. glabrata. Furthermore, we applied a liquid chromatography-tandem mass spectrometry (LC–MS/MS) -based approach for the characterization of β-ME extract and secretory protein extract from C. glabrata, which resulted in a comprehensive cell wall and secretory proteome map.portunistic fungal pathogen of humans, with the emergence of resitant strains being of particulary concern. The cell wall associated and secretory proteins are intrinsically involved in vital functions during the fungal-host interactions. These proteins are responsible for eliciting immune response in host during candidiasis and therefore can be a promising candidates for vaccine development. BALB/c mice were broadly divided into unimmunized, immunized groups and the designated groups were immunized with β-mercaptoethanol (β-ME) call wall extract, and secretory protein extract from C. glabrata via intraperitoneal route. All the animals were then challenged with a lethal dose of C. glabrata. Furthermore, we applied a liquid chromatography-tandem mass spectrometry (LC–MS/MS) -based approach for the characterization of β-ME extract and secretory protein extract from C. glabrata, which resulted in a comprehensive cell wall and secretory proteome map.

INSTRUMENT(S):

ORGANISM(S): Candida Glabrata (yeast) (torulopsis Glabrata)

SUBMITTER: Previn Naicker  

LAB HEAD: Stoyan Stoychev

PROVIDER: PXD032928 | Pride | 2023-03-11

REPOSITORIES: Pride