Personalized phosphoproteomics of skeletal muscle insulin resistance and exercise links MINDY1 to insulin action

ABSTRACT: Insulin resistance, the defective regulation of glucose metabolism by insulin, increases the risk of type 2 diabetes and cardiovascular disease. It is not yet known how insulin resistance remodels signalling networks in human tissues. Here, we define the signalling architecture of insulin resistance in skeletal muscle. We measured phenotypes and phosphoproteomes of insulin resistant and insulin sensitive subjects as they responded to exercise and an insulin infusion, quantifying more than 26,000 phosphosites in 114 skeletal muscle biopsies. Incorporating a temporal component to personalised phosphoproteomics identified signalling linked with the range of insulin sensitivity across the participants.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Skeletal Muscle, Muscle Cell

DISEASE(S): Type 2 Diabetes Mellitus

SUBMITTER: Sean Humphrey  

LAB HEAD: Sean Humphrey

PROVIDER: PXD032948 | Pride | 2024-11-22

REPOSITORIES: Pride