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Cellular proteome and secretome analysis of electrically pulse-stimulated human myotubes

ABSTRACT: In this study, we aimed to investigate alterations in secreted proteins as well as expression profile changes in cellular proteome in human skeletal muscle cells that are triggered by a well-established in vitro model of exercise using electrical pulse stimulation (EPS). Here, skeletal muscle cells isolated from muscle biopsies from seven donors were used. The cells from each donor were then exposed to prolonged EPS (i.e. 10 V, 2 ms, 0.1 Hz for 24 h), and non-stimulated cells from the same donors were used as a control.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tuula Nyman

LAB HEAD: Tuula Nyman

PROVIDER: PXD033025 | Pride | 2022-08-12

REPOSITORIES: Pride

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Insight Into the Metabolic Adaptations of Electrically Pulse-Stimulated Human Myotubes Using Global Analysis of the Transcriptome and Proteome.

Mengeste Abel M AM Nikolić Nataša N Dalmao Fernandez Andrea A Feng Yuan Z YZ Nyman Tuula A TA Kersten Sander S Haugen Fred F Kase Eili Tranheim ET Aas Vigdis V Rustan Arild C AC Thoresen G Hege GH

Frontiers in physiology 20220706

Electrical pulse stimulation (EPS) has proven to be a useful tool to interrogate cell-specific responses to muscle contraction. In the present study, we aimed to uncover networks of signaling pathways and regulatory molecules responsible for the metabolic effects of exercise in human skeletal muscle cells exposed to chronic EPS. Differentiated myotubes from young male subjects were exposed to EPS protocol 1 (i.e. 2 ms, 10 V, and 0.1 Hz for 24 h), whereas myotubes from middle-aged women and men w ...[more]

PMID: 35874526

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