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Fish hydrolysate supplementation prevents stress-induced dysregulation of hippocampal proteins relative to mitochondrial metabolism and neuronal network in mice

ABSTRACT: Over the past decades, stress incidence has dramatically increased in occidental societies. The use of natural resources, like fish hydrolysates, appeared as an interesting strategy to improve stress management. Our previous study demonstrated the anxiolytic effects of a fish hydrolysate supplementation in mice exposed to an acute mild stress, by limiting the stress-induced corticosterone release and modulating expression of some stress-responsive genes. Our work now explores hippocampal protein modulation induced by fish hydrolysate supplementation, in mice submitted to an acute mild stress, with the aim to better elucidate the underlying mechanisms. Hippocampi from the same cohort, Balb/c mice supplemented with fish hydrolysate (300 mg/kg body weight) or vehicle daily for 7 days before being submitted or not to an acute mild stress protocol (4 groups, n=8/group), were subjected to a label-free quantitative proteomics analysis conjugated with gene ontology data mining. Our results demonstrated that an acute mild stress modulated the expression of proteins involved in dopamine metabolism, neuron projection and metabolic process. Moreover, fish hydrolysate supplementation prevented stress-induced dysregulation of proteins relative to mitochondrial pathways and neuronal network. These findings suggest that fish hydrolysate represents an innovative strategy to prevent stress-induced aversive effects and participates in stress management.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Julie Le Faouder

LAB HEAD: Charles PIneau

PROVIDER: PXD033053 | Pride | 2022-05-30

REPOSITORIES: Pride

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Fish hydrolysate supplementation prevents stress-induced dysregulation of hippocampal proteins relative to mitochondrial metabolism and neuronal network in mice

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