Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Marchantia phototropin interactome LC-MSMS

ABSTRACT: To identify interactors of Mpphot, we performed an immunoprecipitation assay using wild-type (WT) Marchantia plants and transgenic Marchantia plants expressing Mpphot-Citrine or free Citrine. The experiment was repeated twice.

INSTRUMENT(S):

ORGANISM(S): Marchantia Polymorpha

TISSUE(S): Plant Cell, Thallus

SUBMITTER: Kazuya Ishikawa

LAB HEAD: Yutaka Kodama

PROVIDER: PXD033077 | Pride | 2022-05-27

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Citrine_13-15_F004958.csv	Csv
	Citrine_13-15_F004958.dat	Other
	Citrine_13-15_F004958.mzid.gz	Mzid
	Citrine_13.RAW	Raw
	Citrine_14.RAW	Raw

Items per page:

1 - 5 of 44

Similar Datasets

LZY-interacting proteins in Arabidopsis

Project description:In many plant species, roots maintain specific growth angles relative to the direction of gravity, known as gravitropic set point angles (GSA). These contribute to the efficient acquisition of water and nutrients. AtLAZY1/LAZY1-LIKE (LZY) genes are involved in GSA control by regulating auxin flow toward the direction of gravity in Arabidopsis. LZYs are plant-specific unknown proteins with no domain for which the function is inferable. To clarify the molecular function of LZYs, we identified the proteins that interact with them by immunoprecipitation coupled with mass spectrometry.

2020-01-09 | PXD016219 | Pride

Proteome of rice shoot apical meristem

Project description:DNA methylation is an epigenetic modification that specifies the basic state of pluripotent stem cells and regulates the developmental transition from stem cells to various cell types. In flowering plants, the shoot apical meristem (SAM) contains a pluripotent stem cell population which generates the aerial part of plants including the germ cells. Under appropriate conditions, the SAM undergoes a developmental transition from a leaf-forming vegetative SAM to an inflorescence- and flower-forming reproductive SAM. While SAM characteristics are largely altered in this transition, the complete picture of DNA methylation remains elusive. Here, by analyzing whole-genome DNA methylation of isolated rice SAMs in the vegetative and reproductive stages, we found that methylation at CHH sites is kept high, particularly at transposable elements (TEs), in the vegetative SAM relative to the differentiated leaf, and increases in the reproductive SAM via the RNA-dependent DNA methylation pathway. We also found that half of the TEs that were highly methylated in gametes had already undergone CHH hypermethylation in the SAM. Our results indicate that changes in DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from harmful TEs.

2020-08-14 | PXD020608 | Pride

Arabidopsis Wdr8 interactors identification

Project description:To identify Arabidopsis Wdr8 interactors, immunoprecipitation associated LC-MS/MS analysis was carried out. Crude proteins extracted from Wdr8-GFP expressing transgenic plants was subjected to the immuno-precipitation assay using GFP antibody magnetic beads (µMACS GFP isolation kit, Miltenyi Biotec). Co-purified proteins were separated by 10% SDS-PAGE gel and stained with SYPRO Ruby (BioRad laboratories). The stained protein bands were excised into several fraction pieces according to protein sizes, and in-gel protein digestion by trypsin was carried out. Purified protein peptides were subjected to LC-MS/MS analysis (LTQ-Orbitrap XL-HTC-PAL system). Collected MS/MS peak spectra was analyzed by the MASCOT server to identify peptide sequence.

2022-02-17 | PXD026063 | Pride

proteome analysis of exosomes from WT CtBP2-HA knock-in and Mut CtBP2-HA knock-in MEF cells

Project description:We prepared two types of MEF cells: one is cells with wild-type CtBP2-HA knock-in and the other is Rossmann fold mutant CtBP2-HA knock-in. We isolated exosomes from those cells with the polymer precipitation method and subjected them to proteome analysis.

2025-08-13 | PXD066696 | Pride

Protein abundance estimation in HeLa cells

Project description:To estimate the amount of proteins in HeLa, whole cell digests were subjected to fractionation by off line reverse phase chromatography at basic pH followed by LC-MS/MS analysis

2016-12-07 | PXD001441 | Pride

Citrus vascular proteomics highlights the role of peroxidases and serine proteases during Huanglongbing disease progression

Project description:The dataset for this project was to identify significantly changing proteins in the vasculature of Washington Navel (Citrus sinensis (L) Osbeck) during Candidatus Liberibacter asiaticus (CLas) infection. The navel trees were graft-inoculated with the Hacienda Heights, CA CLas strain (HHCA) and then sampled ten months after graft inoculation.

2020-09-07 | PXD020703 | Pride

Immunoprecipitation of PTEN coupled Nano-LC−ESI-MS/MS

Project description:Nuclear localization of PTEN is essential for its tumor suppressive role, and loss of nuclear PTEN is more prominent than cytoplasmic PTEN in many kinds of cancers. However, nuclear PTEN-specific regulatory mechanisms were rarely reported. Based on the finding that nuclear PTEN is more unstable than cytoplasmic PTEN. Thus, 293T cells were transiently transfected with plasmid encoding Flag-tagged PTEN along with empty vector, followed by affinity purification using an anti-Flag antibody, and the bound proteins were analyzed by liquid chromatography with tandem mass spectrometry (LC−MS/MS). Here we identify that F-box only protein 22 (FBXO22) induces ubiquitylation of nuclear but not cytoplasmic PTEN at lysine 221, which is responsible for the degradation of nuclear PTEN.

2020-04-22 | PXD017983 | Pride

Mps1(Mph1) Kinase phosphorylates Mad3 to Inhibit Cdc20Slp1 –APC/C and maintain spindle checkpoint arrests

Project description:The spindle checkpoint is a mitotic surveillance system which ensures equal segregation of sister chromatids. It delays anaphase onset by inhibiting the action of the E3 ubiquitin ligase known as the anaphase promoting complex or cyclosome (APC/C). Mads/BubR1 is a key component of the mitotic checkpoint complex (MCC) which binds and inhibits the APC/C early in mitosis. Mps1Mph1 kinase is critical for checkpoint signalling and MCC-APC/C inhibition, yet few substrates have been identified. Here we identify Mad3 as a substrate of fission yeast Mps1Mph1 kinase. We map and mutate phosphorylation sites in Mad3, producing mutants that are targeted to kinetochores and assembled into MCC, yet display reduced APC/C binding and are unable to maintain checkpoint arrests. We chow biochemically that Mad3 phospho-mimics are potent APC/C inhibitors in vitro, demonstrating that Mad3p modification can directly influence Cdc20Slp1-APC/C activity. This genetic dissection of APC/C inhibition demonstrates that Mps1Mph1 kinase-dependant modification of Mad3 and Mad2 act in a concerted manner to maintain spindle checkpoint arrests.

2016-03-31 | PXD003806 | Pride

Enriched vascular proteomics reveals insights to the role of peroxidase and serine proteases during infection with Candidatus Liberibacter asiaticus (CLas) in Washington navel.

2020-04-06 | PXD017480 | Pride

An effector from the Huanglongbing-associated pathogen targets citrus proteases

Project description:The dataset for this project was to identify significantly changing papain-like cysteine proteases during Candidatus Liberibacter asiaticus infection in Navel trees from a Texas citrus grove.

2018-04-16 | PXD008366 | Pride