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Region-specific alterations in the developing synaptosomal proteome in the absence of MET signaling

ABSTRACT: The neocortex and striatum were harvested from conditional Met null male mice (Metfx/fx/nestincre) and wild type littermates at postnatal day 14. Synaptosomes were generated and prepared from each region for quantitative proteomic analyses using isobaric tags for relative and absolute quantitation (iTRAQ).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Corpus Striatum, Neocortex

SUBMITTER: Kristie Lindsey Rose

LAB HEAD: Kristie Lindsey Rose, Kathie Eagleson, Pat Levitt

PROVIDER: PXD033204 | Pride | 2023-03-11

REPOSITORIES: Pride

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Alterations in the Proteome of Developing Neocortical Synaptosomes in the Absence of MET Signaling Revealed by Comparative Proteomics.

Eagleson Kathie L KL Levitt Pat P

Developmental neuroscience 20230307 3

Alterations in the expression of genes encoding proteins involved in synapse formation, maturation, and function are a hallmark of many neurodevelopmental and psychiatric disorders. For example, there is reduced neocortical expression of the MET receptor tyrosine kinase (MET) transcript and protein in Autism Spectrum Disorder (ASD) and Rett syndrome. Preclinical in vivo and in vitro models manipulating MET signaling reveal that the receptor modulates excitatory synapse development and maturation ...[more]

PMID: 36882009

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