Dataset Information

Proteomic profiling of tumor tissue-derived extracellular vesicles in colon cancer

ABSTRACT: Colon cancer is one of the most commonly occurring tumors among both women and men, and over the past decades the incidence has been on the rise. As such, the need for biomarker identification as well as an understanding of the underlying disease mechanism has never been greater. Extracellular vesicles are integral mediators of cell-to-cell communication and offer a unique opportunity to study the machinery that drives disease progression, and they also function as vectors for potential biomarkers. Tumor tissue and healthy mucosal tissue from the colons of ten patients were used to isolate tissue-resident EVs that were subsequently subjected to global quantitative proteomic analysis through LC-MS/MS. In total, more than 2000 proteins were identified, with most of the common EV markers being among them. Bioinformatics revealed a clear underrepresentation of proteins involved in energy production and cellular adhesion in tumor EVs, while proteins involved in protein biosynthesis were overrepresented. Additionally, 53 membrane proteins were found to be significantly upregulated in tumor EVs. Among them were several proteins with enzymatic functions that degrade the extracellular matrix, and two of these, FAP and TMEM2, as well as the ephrin receptor EPHB3, were validated and found to be consistent with the global quantitative results. These stark differences in the proteomes between healthy and cancerous tissue emphasize the importance of the interstitial vesicle secretome as a major player of disease development.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Colon

DISEASE(S): Colon Cancer

SUBMITTER: Proteomics Core Facility

LAB HEAD: Aleksander Cvjetkovic

PROVIDER: PXD033236 | Pride | 2026-06-26

REPOSITORIES: Pride

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	2815_11plex_0miss_SPhuman_norm_final.pdResult	Other
	2815_11plex_0miss_SPhuman_notnorm_final-_1_.msf	Msf
	2991_11plex_0miss_SPhuman_norm_final.pdResult	Other
	2991_11plex_0miss_SPhuman_notnorm_final.msf	Msf
	Fusion_180201_46.raw	Raw

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Publications

Proteomic profiling of tumour tissue-derived extracellular vesicles in colon cancer.

Cvjetkovic Aleksander A Karimi Nasibeh N Crescitelli Rossella R Thorsell Annika A Taflin Helena H Lässer Cecilia C Lötvall Jan J

Journal of extracellular biology 20240206 2

Colon cancer is one of the most commonly occurring tumours among both women and men, and over the past decades the incidence has been on the rise. As such, the need for biomarker identification as well as an understanding of the underlying disease mechanism has never been greater. Extracellular vesicles are integral mediators of cell-to-cell communication and offer a unique opportunity to study the machinery that drives disease progression, and they also function as vectors for potential biomark ...[more]

PMID: 38939898

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